BPTI: Certificate of Analysis

Bovine pancreatic trypsin inhibitor is encoded as a precursor protein of 100 amino acids. The precursor BPTI is cleaved in cells to produce mature BPTI. [Creighton etal (1993), J. Mol. Biol. 232:1176-1196] The mature bovine pancreatic trypsin inhibitor (BPTI) is a 58 amino acid protein. The BPTI gene is expressed in E.coli. The cells are grown in media where the sole nitrogen source is ¹⁵N labeled ammonium sulfate and/or sole carbon source is ¹³C glucose.

Purification and Analysis

The recombinant BPTI is purified by a proprietary procedure that preserves the native structure of the protein. The purified protein is homogenous when analyzed by overloaded SDS gels. The pools of purified protein solution are extensively dialysed against water and lyophilized. The purified protein is 99.9% pure according to amino acid composition analysis.

Storage

We recommend that the dried protein powder be stored at -20° C. 2mM (13mg/ml) solution of the BPTI is also stable for several months at 4° C. The protein solution can be sterilized by filtering through a 0.2µM filter. However, one has to ensure that they use a small sized filter so as to not lose sample volume. If the protein solution is kept sterile, it is stable for several months, even at room temperature.

Conditions

The product described here is for research use only and should not be administered to humans or used for any drug purpose. This product is without warranty other than information specifically provided in this sheet. No other warranty is expressed or implied.