Properties and Analysis

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Human Ubiquitin: Properties and Analysis

Ubiquitin Structure

MET

-

GLN

-

ILE

-

PHE

-

VAL

-

LYS

-

THR

-

LEU

-

THR

-

GLY

LYS

-

THR

-

ILE

-

THR

-

LEU

-

GLU

-

VAL

-

GLU

-

PRO

-

SER

ASP

-

THR

-

ILE

-

GLU

-

ASN

-

VAL

-

LYS

-

ALA

-

LYS

-

ILE

GLN

-

ASP

-

LYS

-

GLU

-

GLY

-

ILE

-

PRO

-

PRO

-

ASP

-

GLN

GLN

-

ARG

-

LEU

-

ILE

-

PHE

-

ALA

-

GLY

-

LYS

-

GLN

-

LEU

GLU

-

ASP

-

GLY

-

ARG

-

THR

-

LEU

-

SER

-

ASP

-

TYR

-

ASN

ILE

-

GLN

-

LYS

-

GLU

-

SER

-

THR

-

LEU

-

HIS

-

LEU

-

VAL

LEU

-

ARG

-

LEU

-

ARG

-

GLY

-

GLY

Ubiquitin Analysis

Ubiquitin Analysis	Entire Protein
Length	76 aa
Molecular Weight	8564.47 m.w.
1 microgram =	116.761 pMoles
Molar Extinction Coefficient	1280
1 A[280] corr. to	6.69 mg/ml
A[280] of 1 mg/ml	0.15 AU
Isoelectric Point	6.79
Charge at pH 7	-0.14

Amino Acid(s)	Number count	% by weight	% by frequency
Charged (RKHYCDE)	24	36.35	31.58
Acidic (DE)	11	15.61	14.47
Basic (KR)	11	17.35	14.47
Polar (NCQSTY)	19	24.92	25.00
Hydrophobic (AILFWV)	24	31.02	31.58

A Ala	2	1.80	2.63
C Cys	0	0.00	0.00
D Asp	5	6.71	6.58
E Glu	6	8.90	7.89
F Phe	2	3.33	2.63
G Gly	6	4.54	7.89
H His	1	1.56	1.32
I Ile	7	9.26	9.21
K Lys	7	10.32	9.21
L Leu	9	11.91	11.84
M Met	1	1.50	1.32
N Asn	2	2.66	2.63
P Pro	3	3.48	3.95
Q Gln	6	8.84	7.89
R Arg	4	7.03	5.26
S Ser	3	3.18	3.95
T Thr	7	8.41	9.21
V Val	4	4.73	5.26
W Trp	0	0.00	0.00
Y Tyr	1	1.83	1.32

B Asx	7	9.38	9.21
Z Glx	12	17.75	15.79
X Xxx	0	0.00	0.00

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