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Maltose Binding Protein: Properties and Analysis
MBP Structure
MKTEEGKLVI |
WINGDKGYNG |
LAEVGKKFEK |
DTGIKVTVEH |
PDKLEEKFPQ |
VAATGDGPDI |
IFWAHDRFGG |
YAQSGLLAEI |
TPDKAFQDKL |
YPFTWDAVRY |
NGKLIAYPIA |
VEALSLIYNK |
DLLPNPPKTW |
EEIPALDKEL |
KAKGKSALMF |
NLQEPYFTWP |
LIAADGGYAF |
KYENGKYDIK |
DVGVDNAGAK |
AGLTFLVDLI |
KNKHMNADTD |
YSIAEAAFNK |
GETAMTINGP |
WAWSNIDTSK |
VNYGVTVLPT |
FKGQPSKPFV |
GVLSAGINAA |
SPNKELAKEF |
LENYLLTDEG |
LEAVNKDKPL |
GAVALKSYEE |
ELAKDPRIAA |
TMENAQKGEI |
MPNIPQMSAF |
WYAVRTAVIN |
AASGRQTVDE |
ALKDAQTRIT |
K |
|
MBP Analysis
| MBP Analysis |
Entire
Protein |
| Length |
371 aa |
| Molecular Weight |
40826.4 m.w. |
| 1 microgram = |
24.494 pMoles |
| Molar Extinction Coefficient |
64720 |
| 1 A[280] corr. to |
0.631 mg/ml |
| A[280] of 1 mg/ml |
1.585 AU |
| Isoelectric Point |
5.22 |
| Charge at pH 7 |
-7.69 |
| Amino Acid(s) |
Number
count |
%
by
weight |
%
by
frequency |
| Charged (RKHYCDE) |
24 |
36.35 |
31.58 |
| Acidic (DE) |
11 |
15.61 |
14.47 |
| Basic (KR) |
11 |
17.35 |
14.47 |
| Polar (NCQSTY) |
19 |
24.92 |
25.00 |
| Hydrophobic (AILFWV) |
24 |
31.02 |
31.58 |
|
| A Ala |
44 |
7.7 |
11.9 |
| C Cys |
0 |
0.0 |
0.0 |
| D Asp |
24 |
6.8 |
6.5 |
| E Glu |
27 |
8.5 |
7.3 |
| F Phe |
15 |
5.4 |
4.0 |
| G Gly |
29 |
4.1 |
7.8 |
| H His |
3 |
1.0 |
0.8 |
| I Ile |
22 |
6.1 |
5.9 |
| K Lys |
37 |
11.6 |
10.0 |
| L Leu |
30 |
8.3 |
8.1 |
| M Met |
7 |
2.3 |
1.9 |
| N Asn |
21 |
5.9 |
5.7 |
| P Pro |
21 |
5.0 |
5.7 |
| Q Gln |
9 |
2.8 |
2.4 |
| R Arg |
6 |
2.3 |
1.6 |
| S Ser |
12 |
2.6 |
3.2 |
| T Thr |
21 |
5.2 |
5.7 |
| V Val |
20 |
4.9 |
5.4 |
| W Trp |
8 |
3.7 |
2.2 |
| Y Tyr |
15 |
6.0 |
4.0 |
|
| B Asx |
0 |
0.0 |
0.0 |
| Z Glx |
0 |
0.0 |
0.0 |
| X Xxx |
0 |
0.0 |
0.0 |
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