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Protein G: Properties and Analysis

Protein G Structure

THR - PRO - ALA - VAL - THR - THR - TYR - LYS - LEU - VAL
ILE - ASN - GLY - LYS - THR - LEU - LYS - GLY - GLU - THR
THR - THR - LYS - ALA - VAL - ASP - ALA - GLU - THR - ALA
GLU - LYS - ALA - PHE - LYS - GLN - TYR - ALA - ASN - ASP
ASN - GLY - VAL - ASP - GLY - VAL - TRP - THR - TYR - ASP
ASP - ALA - THR - LYS - THR - PHE - THR - VAL - THR - GLU

Protein G Analysis

Protein G Analysis Entire Protein
Length 60 aa
Molecular Weight 6518.60 m.w.
1 microgram = 153.407 pMoles
Molar Extinction Coefficient 9530
1 A[280] corr. to 0.68 mg/ml
A[280] of 1 mg/ml 1.46 AU
Isoelectric Point 4.81
Charge at pH 7 -2.23
Amino Acid(s) Number
count		 % by
weight		 % by
frequency
Charged (RKHYCDE) 19 37.20 31.67
Acidic (DE) 9 16.54 15.00
Basic (KR) 7 13.50 11.67
Polar (NCQSTY) 20 34.75 33.33
Hydrophobic (AILFWV) 19 29.74 31.67
A Ala 7 8.23 11.67
C Cys 0 0.00 0.00
D Asp 5 8.78 8.33
E Glu 4 7.76 6.67
F Phe 2 4.36 3.33
G Gly 4 3.96 6.67
H His 0 0.00 0.00
I Ile 1 1.73 1.67
K Lys 7 13.50 11.67
L Leu 2 3.46 3.33
M Met 0 0.00 0.00
N Asn 3 5.23 5.00
P Pro 1 1.52 1.67
Q Gln 1 1.93 1.67
R Arg 0 0.00 0.00
S Ser 0 0.00 0.00
T Thr 13 20.42 21.67
V Val 6 9.27 10.00
W Trp 1 2.69 1.67
Y Tyr 3 7.17 5.00
B Asx 8 14.01 13.33
Z Glx 5 9.69 8.33
X Xxx 0 0.00 0.00

 

  
   

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